Rights: The University of Waikato Published 18 November 2011 Download


Peter Hosking – Living Cell Technologies

The SGS assay is one of our critical quality control parameters. SGS stands for static glucose stimulation, and essentially, the assay is such that it challenges the islet with firstly a low concentration of glucose, then a high concentration of glucose, then a low concentration of glucose again. And so we measure the amount of insulin that is excreted at the low glucose level, then at the high glucose level, and then obviously when there’s no glucose there again, we want the cell to switch off and stop pumping out insulin, and so we also check that that happens. And the SGS assay gives us confidence that the cells that we’re using for patients will in fact produce insulin in the sufficient quantities to cause a good clinical outcome.