Islet cell culture

Transcript

Peter Hosking - Living Cell Technologies

The next step once we’ve isolated the islets is to culture them for 3 days. We do this in spinner flasks, which are about 1 litre, 1.2 litre size. They sit on a magnetic drive and they agitate. And we have in each spinner flask about 300,000 pancreatic islets, and we culture these in about 300 ml of media. We have to change the media a number of times during the 3 days because the islets require oxygen and glucose and they consume some of the nutrients that are in the media. Then after 3 days, we’re ready to proceed to the next phase, which is the encapsulation process.