Fluorescence in-situ hybridisation

Transcript

MARION WOODCOCK
When looking at how many copies are incorporated and where within the genome we use a technique called FISH which is fluorescence in-situ hybridisation, and I will take a biopsy from the animal and grow up cells back into culture and then arrest them at metaphase, prepare some slides with those cells, and then I will probe my slides for the transgene. And on the slides I will have an array of the chromosomes, and where the transgene has incorporated will glow on those chromosomes. So if there is one site of insertion we will see one site where the probe will glow.