Rights: University of Waikato Published 28 April 2010 Download


So what we are doing with ligation is just sticking the gene of interest into an expression vector.

With this example of ligation I have a vector backbone, which is already been opened by a restriction enzyme and has what we call a sticky end. And then I have my gene of interest, which is a short fragment, which also has sticky ends. And then using a ligation enzyme and mixing these two fragments together the sticky ends will be like stuck together that is what a ligation enzyme will do. So that is incubated in the water bath at 16 degrees for half an hour.