Rights: University of Waikato Published 28 April 2010 Download


Designing the gene construct

To design a gene construct we look at what the overall function will be and we then align it all logically. It will require the promoter, the gene of interest, and then a poly A tail. Because we need to amplify up or make more copies of this gene so that we have bulk to work with we have bacterial sequences involved so that it can be amplified within bacteria.

For most of our applications we are designing our construct that they are only active in their mammary gland, so the expression is only at late pregnancy and during lactation, the then protein produced in the mammary gland will then be secreted into the milk.