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    Rights: © Copyright. 2014. University of Waikato. All Rights Reserved. Published 13 March 2014 Size: 38 KB Referencing Hub media

    To clone a stretch of DNA (such as a gene) into a vector, restriction enzymes are used to cut out the DNA of interest and to open up the vector. The DNA is added to the vector by mixing the two together in the presence of the enzyme DNA ligase.